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KMID : 0545120010110020275
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 2 p.275 ~ p.280
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Purification and Characterization of Bacteriocin J105 Produced by Lactococcus lactis subsp. lactis J105 Isolated from Kimchi
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Kwak, Gyu Suk
Kim, Sung Koo/Jun, Hong Ki
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Abstract
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Bacteriocin J105 is a proteinaceous inhibitory substance produced by Lactococcus lactis subsp. lactis J105 isolated from Kimchi. Bacteriocin J105 was purified to homogeneity by the pH-dependent adsorption-desorption method and reverse-phase HPLC from the culture broth of Lactococcus lactis subsp. lactis J 105. Purification of bacteriocin J105 resulted in a 1.47-fold increase in the specific activity and the recovery was 1.5%. Its molecular mass measured by the electrophoretic pattern in the sodium dodecyl sulfate polyacrylamide gel was about 3.4kDa. It was stable at 121℃ for 15min at pH between 2 and 4. However; at pH above 5, bacteriocin was rapidly inactivated. Twenty-one residues from the N-terminal portion of bacteriocin J105 were sequenced using sequence analysis of lantibiotics. Bacteriocin J105 showed significant homology with known nisin A from lactic acid bacteria.
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KEYWORD
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